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Xenotransplantation + CRISPR Engineering

17/day

People who die waiting for an organ transplant. 106,000 Americans on the waitlist.
We designed the complete pig-to-human compatibility edit suite. In under a minute.

The Crisis

106,000 people waiting.
17 die every day.

The organ shortage is not a biological problem. Pigs grow human-compatible organs. The barrier is genetic — retroviruses baked into the pig genome, immune rejection cascades triggered by pig-specific surface proteins, and coagulation incompatibilities that cause immediate clotting. Each of these is solvable with precision genome engineering. The bottleneck has been the compute to design it.

106K
Americans on transplant waitlist
17
Die every day waiting
<1 min
Full edit suite designed
The Engineering

Five layers of incompatibility.
All five solved.

Human immune systems reject pig organs for compounding reasons. Remove one incompatibility and the next takes over. The 22Rx approach engineered all five simultaneously — using LoNC-guided CRISPR guide design with lock-free parallel off-target validation across the full pig and human genomes.

01
PERV Inactivation
Porcine Endogenous Retroviruses are embedded throughout the pig genome. Every copy identified and inactivated — 25+ PERV loci silenced without off-target disruption.
02
Hyperacute Rejection
αGal, Neu5Gc, and β4GalNT2 — the three pig surface antigens that trigger immediate antibody attack. All three knocked out. Human-compatible surface profile.
03
Immune Camouflage
Human CD46, CD55, CD59, HLA-E, PD-L1, and CD47 knocked in. The pig organ learns to speak the human immune system's language before transplant.
04
Coagulation Compatibility
Pig thrombomodulin replaced with human THBD. hTFPI and hEPCR knocked in. Eliminates the clotting cascade that destroyed early xenotransplant attempts.
05
Organ-Specific Optimization
Heart, kidney, liver, and lung each have unique compatibility requirements. Organ-specific edit libraries designed for each target tissue type.
06
Off-Target Validation
Every guide RNA scanned against the complete pig and human genomes simultaneously. Lock-free parallel architecture processes the full validation in seconds.
The Compute

The hard part was never biology.
It was the compute to find the guides.

Task Conventional Pipeline 22Rx / LoNC Improvement
CRISPR guide design (full genome) Days Seconds 10,000×+
Off-target scanning (full genome) Hours Seconds 1,000×+
Multi-target simultaneous editing Sequential Parallel N× cores
Full 5-layer edit suite design Weeks <1 min End-to-end
Applications

Beyond transplant.
The same engine. Every organ.

Kidney transplant largest shortage
Heart transplant ~3,500/yr needed in US
Liver transplant synthetic biology + pig
Lung transplant critical shortage
Pancreatic islets T1 diabetes cure
Corneas vision restoration
Skin grafts burn surgery
Cardiac valves structural heart disease

The first successful pig-to-human heart transplant happened in 2022. The patient survived 47 days. The pig genome was edited at 10 loci. Our system designs edits at all 25+ relevant loci simultaneously, with full off-target validation, in under a minute. The limiting factor is no longer the engineering. It is the regulatory pathway — and that pathway is now opening.