CRISPR cuts.
Base editing rewrites. Prime editing replaces.
Each generation of gene editing improves on the last. CRISPR makes precise cuts. Base editors convert one nucleotide to another without cuts. Prime editors write entirely new sequences into the genome. 22Rx designs guides for all three — simultaneously, in parallel, with full off-target validation against the entire human genome — in seconds.
| Technology | What It Does | Guide Design Time | Off-Target Scan |
|---|---|---|---|
| Standard CRISPR-Cas9 | Double-strand break + HDR or NHEJ | Hours–days | Manual / partial |
| Base Editing (CBE/ABE) | C→T or A→G, no breaks | Hours | Computational (slow) |
| Prime Editing (PE3) | Any edit, any sequence, no breaks | Hours–days | Very limited tools |
| 22Rx / LoNC (all three) | Full guide library — all modalities | Seconds | Full genome, parallel |
Every disease with a
known genetic root cause.
24
Gene targets designed end-to-end
3B
Base pairs scanned for off-targets
0
DNA breaks required (base + prime editing)
01
Sickle Cell Disease
BCL11A silencing reactivates fetal hemoglobin. Same mechanism as Casgevy (first approved CRISPR therapy). Guide designs and in silico off-target scans completed in seconds.
02
Cancer (TP53 / PCSK9)
TP53 pathway correction for tumor suppression. PCSK9 knockout for lifelong LDL reduction — a one-time edit instead of a daily statin.
03
Progeria / Aging
LMNA G608G — the single point mutation that causes Hutchinson-Gilford Progeria. One base edit. C-to-T. No breaks. Children currently die at 14.
04
Cystic Fibrosis
CFTR ΔF508 — deletion of 3 bases. Prime editing writes the correct sequence directly. No CRISPR cut. No donor template. The first genetic correction that doesn't depend on cell repair machinery.
05
Alzheimer's Risk
APOE4 → APOE2 conversion via base editing. APOE4→APOE2 edit designed computationally; risk reduction is a literature-based hypothesis pending clinical validation. Single base change. Off-target profile computed in silico.
06
Fragile X / SMA
FMR1 CGG repeat expansion correction. SMN1 exon 7 splice site correction. Both targeted by prime editing — multi-base corrections that base editors cannot reach.
Every guide scanned.
Full genome. No exceptions.
Whole-genome off-target scan 3B bases, seconds
Chromatin accessibility modeling tissue-specific
Mismatch tolerance profiling 1–5 bp variants
Bystander edit prediction base editing
PegRNA secondary structure prime editing
PAM variant scanning SpCas9 + variants